People


Richard Hrabal      Ivana Bartosova  Vaclav Veverka
                       Jan Lang        Hana Dvorakova

Richard Hrabal
Head of Laboratory
e-mail: hrabalr@vscht.cz

Education and Qualification:
1989 - Ph.D. in organic chemistry ICT in Prague. Thesis: Synthesis, structure and reactivity of highly fluorinated dienes and trienes
1988 - Postgradual course in liquid and solid phase NMR spectroscopy Technical University in Bratislava
1982 - MSc. in organic chemistry Institute of Chemical Technology in Prague. Thesis: Study of fluorinated dienes

Employment:
1996 Head of the NMR laboratory at the ICT Prague
1993-1996 Research Associate at the Biotechnology Research Institute, div. Biomolecular Interactions. National Research Council of Canada
1992-1993 Head of the NMR laboratory at the ICT Prague
1987-1992 Research Assistant in the NMR Laboratory at the ICT Prague
1986-1987 Research Assistant at the Department of Organic Chemistry of the ICT Prague
1990 Short term visit at the Inorganic Chemistry Department of the Oxford University Prof. R.J.P. Williams. (Study of binding zinc ion to short peptides)

Research Interests:
High resolution NMR spectroscopy and its application in chemistry, biochemistry and molecular biology. Molecular modelling, esp. related to the elucidation of structures of petides, proteins and their complexes.

List of recent publications:

    1.  Srinivasan J., Hu S., Hrabal R., Zhu Y., Komives E.A., Ni F.: Biochemistry 33, 13553(1994) Thrombin-Bound Structure of an EGF Subdomain from Human Thrombomodulin Determined  by Tranferred Nuclear Overhauser Effect [Abstract]
    2. Blackmar C., Healy V.L., Narendra U., Hrabal R., Ni F., Komives E.A.: Bioorganic Chemistry 23, 519-527 (1995)  Structure activity of the region of thrombomodulin that binds thrombin
    3. Hrabal R., Komives. E.A., Ni F.: Protein Science, 5, 195 (1996) Structure Resiliency of an EGF-like Subdomain Bound to its Target Protein, Thrombin. [Abstract]
    4.  Hrabal R., Chen Z., James S., Bennet H.P.J., Ni F.: Nature Structural Biology, 3, 747 (1996) The hairpin stack fold, a novel protein architecture for a new family of protein growth factors. [Abstract]
    5.  Fulton D.B., Hrabal R., Ni, F.: J. Biomolecular NMR, 8, 213-218 (1996) Gradient-enhanced TOCSY experiments with improved sensitivity and solvent suppression. [Abstract]
    6.  Lang J., Dvořáková H., Bartošová I., Lhoták P., Stibor I., Hrabal R.: Tetrahedron Lett. 40,  373-376 (1999).Conformational flexibility of a novel tetraethylether of thiacalix[4]arene. A comparison with the "classical" methylene-bridged compounds. [Abstract]
    7.  Popsavin V., Beric O., Popsavin M., Csanadi J., Vujic D., Hrabal R.: Tetrahedron Lett. 40, 3629-3632 (1999). Dioxolane Acetal Ring Expansion during a Sugar Triflate Displacement. Synthesis and Assignment of Diastereoisomer Configuration of Novel 9-Crown-3 Ether Derivatives.
    8.  Radek Pohl, Jan Pawlas, Stanislav Bohm, Richard Hrabal, Hana Dvorakova, Josef Kuthan: Mendeleev Communications 1999, 74-75. Atropisomeric and atropdiastereoisomeric 2-substituted 1-aryl-3,5-diphenylpyrroles.
    9.  Malá Š., Dvořáková H., Hrabal R., Králová B.: Carbohydrate Res. 322, 209-218 (1999). Towards regioselective synthesis of oligosaccharides by use of alpha-glucosidases with different substrate specificity. [Abstract]
    10. Veverka V., Hrabal R., Durchan M, Stys D.: J. Mol. Str., 523/1-3, 281-287 (2000), Studies of phospholipid binding to N-terminal domain of membrane protein light-harvesting complex II [Abstract]

Vaclav Veverka

e-mail: veverkav@vscht.cz

Education and Qualification:
since 1996 Ph.D. student at Laboratory of NMR Spectroscopy, ICT in Prague. Thesis: Study of structure and properties of proteins using NMR Spectroscopy.
1991 - 1996 MSc. in Analytical Chemistry, ICT in Prague, Thesis: Study of complexation of some calix[4]arenes with selected ion pairs using NMR and IR Spectroscopy

Research Interests:
High resolution NMR spectroscopy and its application in supramolecular chemistry, biochemistry and molecular biology ( membrane protein structures, lipid-protein interactions)

List of publications:

  1. "Studies of lipid binding to fragments of membrane protein light-harvesting complex II by NMR spectroscopy", 2nd ICMSB Vienna, 10.-14. Sep. 1997 Austria
  2. "Structure and the lipid binding properties of the lipid binding site of membrane protein light-harvesting complex II", Lund coloid symposium, 22.-23. Oct. 1997, Sweden
  3. "Polysacharides and lipids labeled by stable isotopes in red alga Porphyridium crueltum",  Stable isotope aided NMR of Biomolecules , 4.-7. Apr. 1998, Oxford, United Kingdom
  4. Veverka V., Hrabal R., Durchan M, Stys D.: J. Mol. Str., 523/1-3, 281-287 (2000), Studies of phospholipid binding to N-terminal domain of membrane protein light-harvesting complex II [Abstract]
Hana Dvorakova

e-mail: dvorakoh@vscht.cz

Education and Qualification.
since 1996 Research Assistant at the NMR laboratory at the ICT Prague
1994 One year stay at the Organic Chemistry Department of the University of Leicester (Great Britain),
1991 Ph.D. in organic chemistry Institute of Organic Chemistry and Biochemistry , Academy of Sciences of the Czech Republic, Prague. Thesis: Acyclic Analogues of Nucleosides and Nucleotides with Modified Purine Bases.
1983-1995 Research Assistant at the Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic, Prague
1978 MSc. in organic chemistry ICT in Prague

Research Interests:
Synthesis and characterisation of compounds with antiviral activity based on nucleosides and nucleotides.
Application of high resolution NMR spectroscopy in structural analysis in chemistry and biochemistry,
study of reaction mechanisms and kinetics.

Selected publications:

  1. Dvořáková H., Dvořák D., Holý A.: Coupling of 6-Chloropurines with Organocuprates Derived from Grignard Reagents: A Convenient Route to sec and tert 6-Alkylpurines. Tetrahedron Lett. 37, 1285 - 1288 (1996).
  2. Dvořáková H., Masojídková M., Holý A., Balzarini J., Andrei G., Snoeck R., De Clercq E. : Synthesis of 2´-Aminomethyl Derivatives of N(2-(Phosphonomethoxy)ethyl) Nucleotide Analogues as Potential Antiviral Agents. J. Med. Chem. 39,  3263 - 3268 (1996). [Abstract]
  3. Holý A., Dvořáková H., Jindřich J., Masojídková M., Buděšínský M., Balzarini J., Andrei G., De Clercq E. : Acyclic Nucleotide Analogs  Derived from 8-Azapurines: Synthesis and Antiviral Activity. J. Med. Chem. 39, 4073 - 4088 (1996).  [Abstract]
  4. Blindauer C.A., Holý A., Dvořáková H., Sigel H.: Solution properties of antiviral adenine-nucleotide analogues. The acid-base properties of 9-[2-(phosphonomethoxy)ethyl]adenine (PMEA) and its N1, N3, and N7 deaza derivatives in aqueous solution. J. Chem. Soc., Perkin Trans. 2, 2353 - 2363 (1997). [Abstract]
  5. Blindauer C.A., Emwas A.H., Holý A., Dvořáková H., Sletten E., Sigel H.: Complex Formation of the Antiviral 9-[2-(Phosphonomethoxy)ethyl]adenine (PMEA) and Its N1, N3, and N7 Deaza Derivatives with Copper(II) in Aqueous Solution. Chem.Eur. J. 3, 1526 - 1536 (1997). [Abstract]
  6. Lang J., Dvořáková H., Bartošová I., Lhoták P., Stibor I., Hrabal R.: Conformational Flexibility of a Novel Tetraethylether of Thiacalix[4]arene. A Comparison with the “Classical” Methylene-Bridged Compounds. Tetrahedron Lett. 40, 373 - 376 (1999). [Abstract]
  7. Pihera P., Dvořáková H., Svoboda J.: Diels-Alder Reactions of Vinyl Derivatives of [1]Benzothieno[3,2-b]furan. Collect. Czech. Chem. Commun. 64, 389 - 407 (1999). [Abstract]
  8. Holý A., Gunter J., Dvořáková H., Masojídková M., Andrei G., Snoeck R., Balzarini J., De Clercq E.: Structure-Antiviral Activity Relationship in the Series of Pyrimidine and Purine N-[2-(2-Phosphonomethoxy)-ethyl] Nucleotide Analogues. 1. Derivatives Substituted at the Carbon Atoms of the Base. J. Med. Chem. 42, 2064 - 2086 (1999). [Abstract]
  9. Pohl R., Pawlas J., Bohm S., Hrabal R., Dvořáková H., Kuthan J.: Atropoisomeric and atropodiastereomeric 2-substituted 1-aryl-3,5-diphenylpyrroles. Mendeleev Communicatios, 2, 74 – 75 (1999).
  10. Hocková D., Hocek M., Dvořáková H., Votruba I.: Synthesis and Cytostatic Activity of Nucleosides and Acyclic Nucleoside Analogues Derived from 6-(Trifluoromethyl)purines. Tetrahedron 55, 11109 – 11118 (1999). [Abstract]
  11. Malá Š., Dvořáková H., Hrabal R., Králová B.: Carbohydrate Res. 322, 209-218 (1999). Towards regioselective synthesis of oligosaccharides by use of alpha-glucosidases with different substrate specificity. [Abstract]


Jan Lang
e-mail: lang@karlov.mff.cuni.cz

Education and Qualification:
1989 - 1994 Mgr. studies in the field of Physics of Molecular and Biological Systems, curriculum biophysics at the Faculty of Mathematics and Physics of the Charles University in Prague, Czech Republic
1993 - 1994 stay at the Division of Physical Chemistry of the University of Stockholm, Stockholm, Sweden
1994 graduated at the Charles University with Magister degree
1994-1999 Ph.D. in physics of molecular and biological systems at Charles University
since 1997 research assistant at ICT Prague
2000 post-doctoral fellow with Prof. J. Kowalewski, Stockholm University, Sweden

Research interests:
Application of high resolution NMR spectroscopy for determination of structural and dynamic properties of small and medium organic and biological molecules. Studies of nuclear relaxation and exchange processes.

Publications:

  1. Lang J; NMR Relaxation Studies in Solution: Adenosine Monophosphates - Nickel(II) Complexes Structure, and Trisaccharide Melezitose Dynamics, Diploma thesis, MFF UK, Praha 1994.
  2. Mäler L., Lang J., Widmalm G., Kowalewski J.: Multiple-Field Carbon-13 NMR Relaxation Investigation on Melezitose. Mag. Reson. Chem. 33 (1995) 541-548.
  3. Štěpánek Josef, Kowalewski Jozef, Lang Jan, Mojzeš Peter: Spectroscopic investigation of nickel cation binding with adenine nucleotides: stability and structure of the 1:2 complex with adenosine 5'-monophosphate. J. Biol. Inorg. Chem. 3 (1998), 543-556. [Abstract]
  4. Lang Jan, Chmelová Kateřina, Štěpánek Josef, Kowalewski Jozef, Holý Antonín: Applicability of spectroscopic methods in investigation of transition metal cation binding to adenine mononucleotides and their analogues. J. Mol. Struct. 480-481 (1999), 363-367. [Abstract]
  5. Lang Jan, Dvořáková Hana, Bartošová Ivana, Lhoták Pavel, Stibor Ivan, Hrabal Richard: Conformational Flexibility of a Novel Tetraethylether of Thiacalix[4]arene. A Comparison with the "Classical" Methylene-Bridged Compounds. Tetrahedron Lett. 40 (1999), 373-376. [Abstract]
  6. Lang Jan: NMR study of structure and dynamics of partially rigid organic molecules, Ph.D. Thesis, Charles University Prague, 1999.
  7. Czernek Jiří, Lang Jan, Sklenář Vladimír: Ab Initio Calculations of Spin-Spin Coupling Constants in Anhydrodeoxythymidines.  J. Phys. Chem. A 104 (2000), 2788 -2792. [Abstract]



Ivana Bartosova

Laboratory Assistence

Technicka 5, CZ 166 28 Prague 6, Czech Republic
e-mail: veverkav@vscht.cz
Tel.:+42 2 2435 3805