The Use of Nonspecific Peptides for Proteomic Identification of Low-Molecular-Weight Proteins from Barley by MALDI-TOF Mass Spectrometry
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a Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic, b Vienna University of Technology, Vienna, Austria, c Research Centre for Study of Extractives from Barley and Hop, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
Nonspecific peptides were analyzed for proteomic identification of low-molecular-weight barley proteins either by MALDI-TOF mass spectrometry involving post-source decay analysis or using a MALDI-TOF/TOF instrument. Identification of low-molecular-weight barley proteins was successfully accomplished by MS/MS analysis of both tryptic and nonspecific peptides in the cases where peptide mass fingerprinting failed. Utilization of nonspecific peptides also increased protein sequence coverage in database searching.