A series of novel dimeric distamycin derivatives with increasing number of 1-methylpyrrole units, was synthesized. In comparison with natural distamycin, the dimeric derivatives contain a Tröger base as a bridging unit in the centres of their molecules. The main goal of this work was to study the influence of interaction with calf thymus DNA on the the distamycin derivative molecule by ¹H NMR spectrometry and to determine the binding site. It was observed that addition of DNA caused broadening of hydrogen signals and also changes in corresponding relaxation times. Both facts are in accordance with the assumed interaction. The results are discussed for different derivatives in dependence on the possible binding site and compared with those from circular dichroism.