Effect of foliar application of Se doses (0.5-20 g per hectare) on Se accumulation in winter wheat grain was investigated in field fertilization experiments. Se fertilization (as Na₂SeO₃•5H₂O solution) was realized at the growth stage of the 6^th leaf. Se fertilization did not influence the yields of grain. Both the doses 10 and 20 g Se per hectare significantly increased Se accumulation in grain. The average Se content in dry matter of grain was 0.039 mg per kg without Se treatment; at the Se doses 10 and 20 g per hectare it increased to 0.094 and 0.192 mg per kg, respectively. Hence the dose 10 g Se per hectare is sufficient for reaching the required Se content in winter wheat grain.