The article presents the possibilities of identification of selected antidepressants of new generations in biological materials. Identification of the drugs by TLC and GC-MS after ether extraction from biological materials (urine, stomach content) was tested. The detection is sensitive and reliable for most of the drugs. For quantitative GC-MS analysis, the drugs were isolated from blood or serum by solid phase extraction (SPE). The recovery of most drugs is over 70 %. Limits of detection (LOD, 3 S/N) as well as limits of quantification (LOQ, 10 S/N) have been determined. The LOD are 0.5−2 ng l⁻¹ for citalopram, maprotiline, mianserine, mirtazapine, moclobemide, sertraline and venlafaxine, 15 ng l⁻¹ for fluoxetine and milnacipran and 50 ng l⁻¹ for fluvoxamine, paroxetine and trazodone. LOQ are 2−5 ng l⁻¹ for citalopram, maprotiline, mianserine, mirtazapine, moclobemide, sertraline and venlafaxine, 50 ng l⁻¹ for fluoxetine and milnacipran and 100 ng l⁻¹ for fluvoxamine, paroxetine and trazodone. For all the monitored drugs, the method is sufficiently sensitive in acute intoxications and for most of the drugs also in determination of therapeutic levels.