Proteome analysis is a relatively new providing information about the quantitative changes in protein expression under precisely defined conditions. The procedure should complete data obtained by DNA sequencing and should offer new objectives for biological and medical studies. Likewise DNA sequencing, proteome study is a highthrough-put technology producing huge amounts of data that need to be exchanged. For this purpose, large networked databases of proteins separated by two-dimensional gel electrophoresis are constructed.This paper describes the formation of the first Czech database of this kind which can be used by the Czech scientific community for publishing the results of proteome studies.